Millati, R., Isroi, Ishola, M. M., Syamsiah, S., Cahyanto, M. N., Niklasson, C., Taherzadeh, M. J.
Oil palm empty fruit bunch (OPEFB) was pretreated using white-rot fungus Pleurotus floridanus, phosphoric acid or their combination, and the results were evaluated based on the biomass components, and its structural and morphological changes. The carbohydrate losses after fungal, phosphoric acid, and fungal followed by phosphoric acid pretreatments were 7.89%, 35.65%, and 33.77%, respectively. The pretreatments changed the hydrogen bonds of cellulose and linkages between lignin and carbohydrate, which is associated with crystallinity of cellulose of OPEFB. Lateral Order Index (LOI) of OPEFB with no pretreatment, with fungal, phosphoric acid, and fungal followed by phosphoric acid pretreatments were 2.77, 1.42, 0.67, and 0.60, respectively. Phosphoric acid pretreatment showed morphological changes of OPEFB, indicated by the damage of fibre structure into smaller particle size. The fungal-, phosphoric acid-, and fungal followed by phosphoric acid pretreatments have improved the digestibility of OPEFB’s cellulose by 4, 6.3, and 7.4 folds, respectively.
Oil palm empty fruit bunch (OPEFB) atau minyak kelapa sawit biji kosong diperlakukan terlebih dahulu menggunakan jamur putih busuk Pleurotus floridanus, asam fosfor atau kombinasinya, dan hasilnya dinilai berdasarkan komponen biomassa dan perubahan struktural dan morfologinya. Kehilangan karbihidrat setelah penjamuran, asam fosfor, dan penjamuran diikuti dengan pra-perlakuan asam fosfor, masing-masing yaitu 7,89%, 35,65%, dan 33,77%. Pra- perlakuan ini mengubah ikatan hydrogen selulosa dan ikatan antara lignin dan karbohidrat, yang dihubungkan dengan kristalinitas selulosa dari OPEFB. Lateral Order Index (LOI) dari OPEFB tanpa pra-perlakuan, dengan jamur, asam fosfor, dan jamur diikuti dengan pra-perlakuan asam fosfor, masing-masing yaitu 2,77, 1,42, 0,67, dan 0,60. Pra-perlakuan asam fosfor menunjukkan perubahan morfologis dari OPEFB, ditandai dengan kerusakan struktur serat menjadi ukuran partikel yang lebih kecil. Jamur, asam fosfor, dan jamur yang diikuti pra-perlakuan asam fosfor telah meningkatkan pencernaan selulosa OPEFB, masing-masing yaitu 4, 6,3, dan 7,4.